Morula stage

The results are as follows : ( 1 ) The mitochondria in the embryos before morula stage were mostly hooded .

结果表明：（1）在桑椹期以前，胚胎中大都为带帽线粒体，从此期开始这种线粒体明显减少；

The experiment showed that the sampling periods for calculating the egg-fertilizing rate are clearage stage , whereas , morula stage is best .

本试验通过对四大家鱼鱼卵受精率计算方法的初步研究，得出如下结论：（1）计算家鱼鱼卵受精率的取样时间为卵裂期，尤以桑椹期为佳。

Embryos from fetal fibroblast could only develop to morula stage with the rate of 0.2 % ;

胎儿成纤维细胞重构胚最高发育阶段为桑椹胚，比例为02%；

There was no significant difference in lambing rate per embryo from the morula stage to blastocyst ( P > 0.05 ) .

胚胎成羔率从桑椹胚到囊胚差异不显著（P>0.05）。

Most embryos from natural estrous rats migrated into the uterus ahead of morula stage , while embryos from superovulated rat entered the uterus at 8-cell stage .

自然发情大鼠的胚胎多在近桑椹胚期进入子宫，而超排大鼠在8-细胞期即已进入子宫。

Gap junctions formed at the 4-cell stage ; while tight junctions and desmosomes appeared at the 8-cell stage simultaneously . The embryos became compact at the morula stage .

间隙连结最早形成于4细胞期，紧密连结和桥粒分别从8细胞期开始出现。

The development of reconstructed embryos was observed . 51.33 % of the reconstructed embryos can develop to 2-cell stage , 31.42 % can develop to 4-cell stage , but only 9.73 % can develop to morula stage .

通过体外培养观察，2-细胞胚胎发育率可达51.33%，4-细胞发育率为31.42%，但发育至桑椹胚阶段的胚胎数目大大减少，仅为9.73%。

Results : ( 1 ) Development from two cell embryos to either morula or blastocyst stage was inhibited by ropivacaine at the highest concentration 100 μ g / ml , and by lidocaine at a concentration as low as 1 μ g / ml.

结果：罗比卡因只在高浓度100μg/ml时对2-细胞至囊胚期的胚胎发育产生明显抑制影响。利多卡因则在1μg/ml的浓度即对2-细胞期胚胎发育产生明显抑制。

Co-culture with somatic cells resulted in significantly more zygotes cleaved ( 18.5 % ) and developing to the morula and blastocyst stage ( 8.6 % ) compared with the somatic cell-free culture ( 9.6 % and 0 % , P < 0.05 ) .

体外受精后采用体细胞共培养的分裂率和桑椹胚／囊胚发育率分别为18.5%和8.6%，显著高于非共培养系统（9.6%和0，P＜0.05）。